Application of a novel high resolution widefield surface plasmon microscope in cell engineering, wound healing and development of new binding assays

Surface Plasmon (SP) microscope systems are mostly built around the prism based Krctschmann configuration. In thcsc systcms the generation of Surface Plasmons (SPs) is achieved by p-polarized light striking a metallised prism surfacc at a spccific angle and then monitoring thc intensity of the reOected light. Thus in these systems. an image of the material can be obtained in terms of an intensity map. in which the intcnsity of thc image is dcpendent on the way the light couples into the SPs. The drawback of these systems is that lateral resolution relies on the ability of plasmons to propagate along the metallised layer. The lateral resolution is thus limited to a few microns. Therefore, a new microscope systcm was developed. i.e. thc Widcficld Surface Plasmon Resonance (WSPR) microscope. that is not only capable of analysing molecular interactions at high vertical resolutions. but also enables SP imaging at much higher lateral resolution than prism based systcms. The functionality of thc novel (WSPR) microscope has been investigated by imaging a scquence of binding events between micropattcrncd cxtracellular matrix proteins and their specific antibodies both in air and real-time. Using the WSPR systcm a changc in contrast was observed with each protein binding cvcnts. Images produced via the WSPR system were analyzcd and comparcd qualitatively and quantitatively. The preliminary results acquired for these binding studics between antibody/antigens dcmonstrate that the WSPR systcm capablc of resolving features down to 260nm although the theoretically proven lateral resolution of the WSPR system is -500nm. Cell surface interactions undcr two diffcrent culture conditions. i.e. HaCaTs cultured on SPR substrate with Transforming Growth Factor ~3 (TGF~3) (50ng/lII/) and without TGF~3 were also invcstigated. It was found that I-IaCaTs cultured in the presence of TGF~3 showed enhanced division and motility along with decreased cell attachmcnt as compared with cclls maintained in TGF~3 free media. It is believed that cellular signalling by TGF~3 is very important for enhancing tissue development in wound rcpair. It is confirmed that the WSPR microscope described here can be used to study sequential monomolecular layer of antibody/antigen interactions binding cvents and examination of cell surface intcrfacial intcractions at latcral scales of less than one micron without the need for traditional immunoOuorescent labelling. These rcsults have significant implications in the developmcnt of ncw brecd fast binding assays system and in enabling high resolution detailed examination of the cell surface couplings and ccll signalling proccsses involvcd in cell attachmcnt and migration

Influence of artocarpus altilis fruit extract on cancer cell

Conventional chemopreventive agents causes adverse side effects in cancer patients. Thus, this study focuses on the effects of natural plant extract against cancer cell as a way to reduce or detrimental effects of orthodox drugs. The present study emphasizes on anti-cancer potentiality of Artocarpus altilis fruit extract against cervical cancer cell. Cervical cancer cell treated with methanol extract of artocarpus altilis fruit with the concentration varied from 10μg/ml, 20μg/ml, 30μg/ml, 40μg/ml and 50μg/ml. Treated and untreated (cells without treatment) cell proliferation and half maximal inhibitory concentration IC50 measured after 72 hours of incubation. Cells without treatment, 10μg/ml, 20μg/ml, 30μg/ml, 40μg/ml and 50 μg/ml of extract concentration showed 1.3, 0.98, 0.72, 0.65, 0.51, and 0.3 of cell proliferation factor accordingly with 40μg/ml of IC50 value. The result shows that artocarpus altilis is capable in opposing cervical cancer cell proliferation or growth as the proliferation factor decreases with the increasing dose of extract concentration which can be attributed to the presence of bioactive compound in Artocarpus altilis

Elderly care monitoring system with IoT application

Falls among elderly can pose serious consequences such as injury or even fatal ones. Therefore, it is essential that fall are detected early and away to that is by using IoT platform. The authors have been developing a wearable device for elderly monitoring system utilizing accelerometer. The data from accelerometer is connected to an Internet-of-Things (IoT) platform called ThingSpeakTM. Based on IoT platform, elderly patients can be remotely monitored as long as the care providers have good internet access. The paper presents the experimental results of determining the sensitivity and specificity of the accelerometer used in the proposed system. This is the first step for developing an accurate data acquisition for monitoring purposes. Based on the experimental results, the average percentage for sensitivity obtained for this device is 73.3%, while the average for specificity obtained is 89.3%. Both sensitivity and specificity tests shows promising results which indicates that the device only has a fail rate of 26.7% and error rate of 10.7%

Artocarpus altilis extract effect on cervical cancer cells

This paper elucidate on the effects of Artocarpus Altilis Pulp part on cervix HeLa cancer cell. IC50 values of pulp extract were determined on HeLa cell with different concentration (12.5µg/ml, 25µg/ml, 50µg/ml, 100µg/ml). Cell viability and cell growth were observed up to 72 hours with comparative to control cells. The results obtained in this research quantitatively revealed the dependence of cell proliferation on extract concentration. Control, 12.5µg/ml, 25µg/ml, 50µg/ml, 100µg/ml of concentration showed 100%, 90%, 80%, 50%, 44% cell viability after 72 hours in culture respectively. This study result demonstrates that Artocarpus Altilis has the ability to inhibit cervical cancer cell proliferation

Development of novel home automation system via Raspberry Pi

Automation is not a new idea in our modern life. Large businesses and wealthy homeowners have used this technology for years now [1]. This automation concept can benefit our daily lives through many ways. The aim of this project is to develop a Home Automation System that focused on helping handicapped and elderly people to perform their chores routine such as turn on or turn off fan and light, so that it can be done wirelessly by using handheld devices such as Smartphone. This system will be based on Raspberry Pi, and is designed to be an affordable and reliable home automation system yet, easy to setup and use. It is hoped that it will provide a better living environment, and also to reduce wastage of electricity by giving user the power to control, conserve and react according user needs, that can be done using the scheduling function for automatic operation of home appliances

Optimization of Pulse Duration Parameter for Hela Cells Growth Rate

To introduce a cell or tissue with a gene or DNA, electroporation parameter plays the most important role. Research on electroporation parameters is still in its early stages. Different researches have used different parameters while performing their research. Electroporation is a mechanism of temporarily disrupting the bilayer membrane, in such a way that a hydrophilic pore is established, allowing a pathway into the cell for molecules such as DNA, which then heals up, once again protecting the cell from the outside. There are many theories as to how this can occur, but the simplest view is that a short pulse creates an increase in the trans-membrane potential which if it exceeds a certain threshold (dependent on size and shape of cells) can lead to a thinning of the bilayer, either due to a local dislocation in the membrane. In this study, cervical cancer cells (HeLa cells) was used to expose to single pulse electric field. Thus, for the purpose of this study, the field strength of 1kV/cm was selected and the pulse duration was varied (30µs, 70µs, 100µs, 200µs, 300µs and 600µs) to obtain higher proliferation for a growth rate of HeLa cells. From this study, it is determined that, HeLa cell exposed to 1kV/cm with a pulse duration of 100µs and single pulse revealed the highest and fastest percentage confluence when compared to growth rate of HeLa cell exposed for 30µs, 70µs, 200µs, 300µs and 600µs pulse duration

Histogram Equalization with Filtering Techniques for Enhancement of Low Quality Microscopic Blood Smear Images

This paper presents image enhancement and filtering techniques for microscope blood smear image, in order to improve low image quality that have characteristics: blurred, the diminished true color of objects which are cells , unclear boundary and low contrast between the cells and background. Therefore in this paper proposed histogram equalization (HE) technique followed with filtering techniques such as median filter. HE utilizing to adjust the contrast which based on intensity pixels values, hence able to measure image quality through image histogram as shown in results, while removing noise from the images using filtering and gamma correction parameter in order to distinguish between background and foreground (cells) to get clear borders also. These techniques have been implemented on 46 blood samples. The proposed method successfully improve the readability of the cells in the low quality of blood smear images this mean that contain more information with a good effectiveness which lead for the correct sickness detection and data analysis

Locomotors Rehabilitation System via Gait Analysis with Load cell, Gyroscope and Accelerometer Sensor

Locomotors Rehabilitation System (LRS) has a potential used with the advancement in electronic and computer. It requires sensors for a locomotion measurement and units to communicate between patients and the doctors. These promote a flexible and economical solution to a Gait analysis by develops units to differentiate the abnormal and normal patient different walking pattern. The LRS consist of a PIC microcontroller, RF transceiver, analogue multiplexer plus sensors for the compressive force, acceleration and angular velocity measurement. Later, these measurements are sent to the computer for further human locomotion analysis. The data transmission is optimized up to 250 meters line of sight with ±3 g acceleration, tilt angle at ± 0.1 ° and ≤ 150 Kg bodyweight measurement. The LRS is expected to offer more information than the Gait analysis and also the ability to improve the clinical and rehabilitation applications

Investigation on anti-proliferation properties of porcupine bezoar (hystrix brachyuran) extracts exposed on hela cells lines combined with electroporation technique

Electroporation (EP) is a technique whereby the biophysical changes on the cells that induced external high-intensity electrical field pulses in order to enhance applications in the medical field. It is a molecular biology technique in order to create pores through a cell wall membrane, boost the permeability of the cell membrane, and support chemicals, drugs or DNA to be imported into Hela cells. While by combining electroporation (EP) technique with porcupine bezoar (PB) extract might reduce the proliferation of HeLa cells because this compound extract has the ability of anti-proliferation and also anti-angiogenesis properties for controlling cancer cell growth. This research concentrate on reviewing and analyses the basic concepts and methods of combining electroporation and porcupine bezoar (PB) extract as applied in cancer treatment application. The combination of this technique might be a new alternative for anti-cancer treatment. The combination of this technique might be a new way for anti-cancer treatment. © 2018 Universiti Teknikal Malaysia Melaka. All rights reserved

Combined effect of neolamarckia cadamba leaves and electroporation method on hela cell anti- proliferation process

This study suggests that natural sources may become an important tool in treating cancer. Neolamarckia cadamba (NC) leaves also well-known as “Anthocephalus Cadamba”, is a precious plant in Ayurvedic medicine. HeLa cells are one of the examples of eukaryotic cells type. It is derived from human cervical cancer cells. This experiment is conducted in different concentrations of NC Leaves (1μg/ml, 5μg/ml, 10μg/ml, 20μg/ml, 30μg/ml, 40μg/ml, 50μg/ml, 60μg/ml, 70μg/ml, 80μg/ml, 90μg/ml and 100μg/ml) for 48 hours. This experiment’s result proves that the anti-cancer properties of the extract of NC leaves are by increasing the concentration of extract, the numbers of cell viability will decrease. For contribution, the process of NC leaves extract will be combined with the electroporation process to investigate the effect on HeLa cell. Electroporation parameters used for this study were (voltage 600v/cm, pulse duration 5ms, single pulse)